Changes in SAM2 expression affect lactic acid tolerance and lactic acid production in Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Changes in SAM2 expression affect lactic acid tolerance and lactic acid production in Saccharomyces cerevisiae
BACKGROUND The great interest in the production of highly pure lactic acid enantiomers comes from the application of polylactic acid (PLA) for the production of biodegradable plastics. Yeasts can be considered as alternative cell factories to lactic acid bacteria for lactic acid production, despite not being natural producers, since they can better tolerate acidic environments. We have previous...
متن کاملGSF2 deletion increases lactic acid production by alleviating glucose repression in Saccharomyces cerevisiae
Improving lactic acid (LA) tolerance is important for cost-effective microbial production of LA under acidic fermentation conditions. Previously, we generated LA-tolerant D-LA-producing S. cerevisiae strain JHY5310 by laboratory adaptive evolution of JHY5210. In this study, we performed whole genome sequencing of JHY5310, identifying four loss-of-function mutations in GSF2, SYN8, STM1, and SIF2...
متن کاملImprovement of lactic acid production in Saccharomyces cerevisiae by cell sorting for high intracellular pH.
Yeast strains expressing heterologous L-lactate dehydrogenases can produce lactic acid. Although these microorganisms are tolerant of acidic environments, it is known that at low pH, lactic acid exerts a high level of stress on the cells. In the present study we analyzed intracellular pH (pHi) and viability by staining with cSNARF-4F and ethidium bromide, respectively, of two lactic-acid-produc...
متن کاملThe effect of pyruvate decarboxylase gene knockout in Saccharomyces cerevisiae on L-lactic acid production.
A plant- and crop-based renewable plastic, poly-lactic acid (PLA), is receiving attention as a new material for a sustainable society in place of petroleum-based plastics. We constructed a metabolically engineered Saccharomyces cerevisiae that has both pyruvate decarboxylase genes (PDC1 and PDC5) disrupted in the genetic background to express two copies of the bovine L-lactate dehydrogenase (LD...
متن کاملL(+) lactic acid production and separation from dairy wastes(whey):in situe separation of lactic acid using lon-exchange resins in automatic control of PH.
Whey with a large amount of BOD(50000 PPM) is a dangerous environmental pollutant.this important source of lavtose(4-5%) is A USEFULL SUBSTRATE FOR A RANGE of fermentation processes.lacitic acid with swvwral applications in industries is one of these products.Specially L(+) isomer of this acid worthing 10 times as much as the mixture of L&D,is used in medical purposes such as absorbable surgica...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2014
ISSN: 1475-2859
DOI: 10.1186/s12934-014-0147-7